Normal pedel is server-side Python/C++, here it is JS. Strategising for more responsive element...
The PCR starting point is cool, but best just make a modal calculator.
Roghly predict the mutation load from a PCR reaction based on the template
and yields or predict a target template concentration to aim for a given
Do note that this is often off due to a variety of factors and should not be a replacement for a test library, especially for manganese mutagenesis.
If the PCR has not been done yet, expect around 1,000 ng yield for a 25 µl PCR reaction —the PCR yields with Mutazyme are generally lower than with Phusion, so if you have a value with a different polymerase underestimate from that.
To predict how many doubling one could get it is often handy to know the
theoretical maximum aDNA yield from a PCR. As is clear from a QPCR curve,
a PCR does not run forever and stops after some cycles, either the primers
or dNTPs are finished.
This is a rough guess for various reasons.