Matteo just upgraded both Bootstrap and Font Awesome, and did a major change to the Mako templating so bugginess is a given.
Engineering better proteins is a multifaceted task. Here you will find
an assortment of tools that will help you find a better variant at every
step of the way.
There are many technical terms in protein design and engineering, so if
you are struggling with terminology do check out the glossary and FAQ page.
Primers — Mutant primers:
a sequence and list of desired mutations it will return primers to make each of those mutations.
Primers — Deepscan:
Given a sequence and a codon, say GCT for alanine scanning, it will return
a list of all primers needed to scan the sequence with that mutation
epPCR — Mutantcaller:
Given a sequence and an ab1 trace it will return all mutations present
and also list heterozygous sites (say you have a mixed population or you
pooled two or three variants to cut down on sequencing) epPCR —
Given a sequence and a list of sampled variants, it will say how biased the library
epPCR — Pedel:
Given a sequence, mutational spectrum and mutational load, it will say
how redundant is the library. epPCR —
Given a sequence, mutational spectrum and mutational load, it will say how redundant is the
library from an amino acid point of view.
epPCR — Chances:
Given a sequence and a desired mutation, it will say what are the chances
that mutation ought to have been seen. epPCR —
Given a sequence and mutational bias data, it generate mutations.
Glue — Glue:
Calculates how complete a library is from a coupon collectors problem
standpoint. Glue — GlueIT:
completeness of a library mutated at 1–6 codons.
Calculates the probability of cross-over events QQC (Quick quality
Given an ab1 file of a sequence pool mutated at a codon, it gives the distribution
of nucleotides and codons.
Given a series of Rosetta pmut_scan outputs it shows the mutational landscape
of that protein
A tool to help you pick
the best degenerate codons. Also found in any page that requires codons as a sequence.
This is a revamp and expansion of the old server by Wayne Patrick and
Andrew Firth found Here