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Chances of a mutation

Input

Sequence

In frame sequence that was mutagenised. Note that all symbols that aren't uppecase ATUGC, will be discarded along with a Fasta header (e.g. '>T. maritima Cystathionine β-lyase'), therefore for masked sequences use lowercase.

Sequence Retrieve previous


Library size

Nucleotide mutation matrix

(non-negative numbers. Overall scaling is unimportant as this is taken from the 'mean number of substitutions per daughter sequence' parameter.)
To
From  
A T G C
A
T
G
C



Values are
Mutation load
Mutations
Distribution

PCR cycles

PCR efficiency



For a note about Poisson vs. PCR distribution see here.